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Oklahoma State University

Josh D. Ramsey

Associate Professor, Anadarko Faculty Fellow and Undergraduate Program Director

josh.ramsey@okstate.edu

For research web page click here.

 

ACADEMIC BACKGROUND

PhRMA Foundation Fellow, University of Kansas, 2007

Ph.D., Chemical  & Biomolecular Engineering, University of Illinois, 2006

M. S.,  Chemical Engineering, University of Illinois, 2003

B. S.,  Chemical Engineering, Oklahoma State University, 2000

 

MAJOR AREAS OF RESEARCH

Engineering Novel Gene Delivery Vectors

Improving Industrial Relevant Enzymes and Microorganisms

Microbial Deterioration of Concrete Infrastructure

Virtual Design and Screening of Therapeutic Compounds

 

RECENT RESEARCH ACTIVITIES

Engineering Novel Gene Delivery Vectors

Safe and efficient delivery of genetic material remains a serious challenge limiting the field of gene therapy.  Our group is interested in developing novel methods for gene delivery.  We are currently funded by the American Heart Association to investigate hybrid viral/synthetic gene delivery vectors to target vascular tissue and treat critical limb and myocardial ischemia.

 

Improving Industrial Relevant Enzymes and Microorganisms

Directed evolution is a technique that has been used to successfully re-engineer enzymes for a variety of industrial applications.  Such enzymes show enhanced or novel substrate activity, improved stability in organic solvents, or greater thermostability.  Our lab is interested in using this technique to produce novel enzymes and microorganisms that meet the growing demand for industrially relevant enzymes and microorganisms.

 

We are currently funded by the Oklahoma Transportation Center to investigate microbial degradation of concrete infrastructure.  The microbes that we are studying are believed to produce an acid that attacks concrete and may shorten the lifespan of our concrete infrastructure.

 

RECENT PUBLICATIONS
N.H. Flynn, Ç. Ö. Topal, R.S. Hikkaduwa Koralege, S. Hartson, A. Ranjan, J. Liu, C. Pope, and J.D. Ramsey, “Effect of Cationic Grafted Copolymer Structure on the Encapsulation of Bovine Serum Albumin.” Material Science and Engineering C, Published online 2 Feb 2016.

K. Sahoo, R. Hikkaduwa Koralege, N. Flynn, S. Koteeswaran, P. Clark, S. Hartson, J. Liu, J.D. Ramsey, C. Pope, and A. Ranjan, “Nanoparticle Attachment to Erythrocyte via the Glycophorin A Targeted Ery1 Ligand Enhances Binding without Impacting Cellular Function.” Pharmaceutical Research, Published online 26 Jan 2016. 

G. Premaratne, R. Nerimetia, R. Matlock, L. Sunday. R. Hikkaduwa Koralege, J.D. Ramsey, and S. Krishnan, “Stability, Scalability, and Reusability of a Volume Efficient Biocatalytic System Constructed on Magnetic Nanoparticles.” Catalysis Science & Technology, Published online 24 Nov 2015. 

C. Pope, C. Uchea, N.H. Flynn, K. Poindexter, L. Geng, W.S. Brimijoin, S. Hartson, A. Ranjan, J.D. Ramsey, and J. Liu, “In Vitro Characterization of Cationic Copolymer-Complexed Nanoparticles of Human Butyrylcholinesterase.” Biochemical Pharmacology, 98 (3), 531-539, 2015.

J.D. Ramseyand N.H. Flynn, “Cell-Penetrating Peptides Transport Therapeutics into Cells.” Invited Review, Pharmacology & Therapeutics, 154, 78-86, 2015. 

S. Nasrazadani, R. Eghtesad, E. Sudoi, S. Vupputuri, T. Ley, and J.D. Ramsey, “Application of Fourier Transform Infrared Spectroscopy to Study Concrete Degradation Induced by Biogenic Sulfuric Acid.” Materials and Structures. 1-10, 2015. 

N. Flynn and J.D. Ramsey. Virus-Like Particles in Gene, Vaccine, and Therapeutic Delivery. In Nanoparticles for Biotherapeutic Delivery (Vol. 1). London, UK: Future Science Ltd. 38-48, 2015.

A.S. Nigatu, N. Flynn, S. Vupputuri, and J.D. Ramsey, “Effects of Cell-Penetrating Peptides on Transduction Efficiency of PEGylated Adenovirus.” Biomedicine and Pharmacotherapy, 71 (2015), 153-160, 2015.  

S. Vupputuri, B. Fathepure, G. Wilber, S. Nasrazadani, T. Ley, J.D. Ramsey, “Characterization of an Acid-Producing Microorganism Collected from a Deteriorating Bridge Site.” International Biodeterioration & Biodegradation, 97 (Jan.-Feb.), 128-134, 2015.

G. Kupgan, D. Hentges, N. Muschinske, W.D. Picking, W.L. Picking and J.D. Ramsey, “The Effect of Fiber Truncations on the Stability of Adenovirus Type 5.” Molecular Biotechnology, 56 (11), 979 – 991, 2014. 

S.P. Choudhari, K. Pendleton, J.D. Ramsey, T. Blanchard and W.D. Picking, “A Systematic Approach Toward Stabilization of CagL, a Protein Antigen from Helicobacter pylori that is a Candidate Subunit Vaccine.” Journal of Pharmaceutical Sciences, 102 (8), 2508 – 2519, 2013. 

N.A. Alhakamy, A.S. Nigatu, C.J. Berkland and J.D. Ramsey, “Gene Delivery Applications of Noncovalently Associated Cell-Penetrating Peptides.” Therapeutic Delivery, 4 (6), 741 – 575, 2013.

E. Whitebay, B. Neely, K.G. Gasem and J.D. Ramsey, “In Silico Prediction of Mechanism of Action for Cancer Therapeutics.” Molecular Informatics, 32 (8), 735 – 741, 2013. 

A. Nigatu, S. Vupputuri, N. Flynn, B.J. Neely and J.D. Ramsey, “Evaluation of Cell Penetrating Peptide-Adenovirus particles for Transduction of CAR-Negative Cells.” Journal of Pharmaceutical Sciences, 102 (6), 1981-1993, 2013.

S. Vupputuri, S. Karode, B.J. Neely and J.D. Ramsey, “Protein Impurities from Cell Culture Dramatically Impact Transduction Efficiency of Polymer/Virus Hybrid Vectors.” Journal of Virological Methods, 92 (2013), pp. 1-11, 2013.

K. Singarapu, I. Pal, and J.D. Ramsey, “Modified Polyethylenimine Used to Enhance Adenovirus Gene Delivery.” Journal of Biomedical Materials Research: Part A, 101A, 1857 – 1864, 2013.  

I. Pal and J.D. Ramsey, “The Role of the Lymphatic System in Vaccine Trafficking and Immune Response.” Advanced Drug Delivery Reviews, 63 (10-11), 909-922, 2011.